Anti-RhD antibody [D7C2] (ab281912)
![Anti-RhD antibody [D7C2] (ab281912)](https://www.abcam.com/ps/products/281/ab281912/Images/ab281912-1-AntiRhD-antibody-immunohistochemistry-K562-cells.jpg "Anti-RhD antibody [D7C2] (ab281912)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [D7C2] to RhD
- Suitable for: ICC
- Reacts with: Human
Overview
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Product name
Anti-RhD antibody [D7C2]
See all RhD primary antibodies -
Description
Human monoclonal [D7C2] to RhD -
Host species
Human -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human RhD. Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r). Isolated peripheral lymphocytes were transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
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Positive control
- ICC: K562 cells.
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General notes
ab281912 is the recombinant human IgM version of ab281911.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
D7C2 -
Isotype
IgM -
Light chain type
lambda -
Research areas
Images
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Immunocytochemistry - Anti-RhD antibody [D7C2] (ab281912)
This data was developed using the chimeric version (ab281911).
Immunofluorescence analysis of paraformaldehyde fixed K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) cells labelling RhD with ab281911 at 10 µg/ml for 1hour, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/top-left). Image showing membrane staining. The nuclear counter stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody. K562 cells were immobilized on Shi-fix™ coverslips.
