Anti-RFC4 antibody [EPR14556] - BSA and Azide free (ab250587)
![Anti-RFC4 antibody [EPR14556] - BSA and Azide free (ab250587)](https://www.abcam.com/ps/products/250/ab250587/Images/ab250587-405983-anti-rfc4-antibody-epr14556-bsa-and-azide-free-western-blot.jpg "Anti-RFC4 antibody [EPR14556] - BSA and Azide free (ab250587)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14556] to RFC4 - BSA and Azide free
- Suitable for: ICC, IP, WB
- Reacts with: Human
Overview
-
Product name
Anti-RFC4 antibody [EPR14556] - BSA and Azide free
See all RFC4 primary antibodies -
Description
Rabbit monoclonal [EPR14556] to RFC4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab250587 is the carrier-free version of ab182145. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250587 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14556 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-RFC4 antibody [EPR14556] - BSA and Azide free (ab250587)All lanes : Anti-RFC4 antibody [EPR14556] (ab182145) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : K562 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Predicted band size: 40 kDaThis data was developed using ab182145, the same antibody clone in a different buffer formulation.
-
![Immunoprecipitation - Anti-RFC4 antibody [EPR14556] - BSA and Azide free (ab250587)](https://www.abcam.com/ps/products/250/ab250587/Images/ab250587-2-anti-rfc4-antibody-epr14556-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-RFC4 antibody [EPR14556] - BSA and Azide free (ab250587)This data was developed using ab182145, the same antibody clone in a different buffer formulation.Detection of Human RFC4 by Western Blot of Immunoprecipitate. HeLa whole cell lysate loaded, ab182145 used for blotting immunoprecipitated RFC4 at a 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used at a 1/1500 dilution. -
![Immunocytochemistry - Anti-RFC4 antibody [EPR14556] - BSA and Azide free (ab250587)](https://www.abcam.com/ps/products/250/ab250587/Images/ab250587-1-anti-rfc4-antibody-epr14556-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-RFC4 antibody [EPR14556] - BSA and Azide free (ab250587)This data was developed using ab182145, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HepG2 cells(right) using anti-RFC4 Alexa Fluor® 555 RabMAB and HepG2 cells (left) using Dapi (blue) -
![Anti-RFC4 antibody [EPR14556] - BSA and Azide free (ab250587)](https://www.abcam.com/ps/products/250/ab250587/Images/ab250587-4-benefits-of-recombinant-antibodies.png)
Anti-RFC4 antibody [EPR14556] - BSA and Azide free (ab250587)