Anti-Retinoic Acid Receptor alpha antibody (ab231896)
Key features and details
- Rabbit polyclonal to Retinoic Acid Receptor alpha
- Suitable for: WB, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Retinoic Acid Receptor alpha antibody
See all Retinoic Acid Receptor alpha primary antibodies -
Description
Rabbit polyclonal to Retinoic Acid Receptor alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Retinoic Acid Receptor alpha (C terminal) conjugated to keyhole limpet haemocyanin. Two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.
Database link: P10276 -
Positive control
- ChIP: NB4 cells. WB: HEK-293T cells transfected with a RARA construct lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Retinoic Acid Receptor alpha antibody (ab231896)All lanes : Anti-Retinoic Acid Receptor alpha antibody (ab231896) at 1/750 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a negative control construct
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a RARA constructDilution buffer: BSA/PBS-Tween.
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ChIP - Anti-Retinoic Acid Receptor alpha antibody (ab231896)ChIP assays were performed using NB4 cells, ab231896 and optimized primer pairs for qPCR. Sheared chromatin from 6 million cells and 4 µl of antibody were used per ChIP experiment. QPCR was performed using primers specifc for the TGM2, HMHA1, PRAM1 and H2B genes.
