Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Resistin antibody [EPR23334-171] (ab275878)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23334-171] to Resistin
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Resistin antibody [EPR23334-171]
    See all Resistin primary antibodies

  • Description

    Rabbit monoclonal [EPR23334-171] to Resistin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U937 whole cell lysate; Human white fat tissue lysate; Recombinant human Resistin protein. Flow Cyt: U937 cells. IP: U937 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Resistin antibody [EPR23334-171] (ab275878)
    Western blot - Anti-Resistin antibody [EPR23334-171] (ab275878)
    All lanes : Anti-Resistin antibody [EPR23334-171] (ab275878) at 1/1000 dilution

    Lane 1 : U937 (human histiocytic lymphoma monocyte) whole cell lysate
    Lane 2 : THP-1 (human monocytic leukemia monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:16532325, 14550293).

    Low expression: THP-1 (PMID:16532325 ).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

     

  • Flow Cytometry - Anti-Resistin antibody [EPR23334-171] (ab275878)
    Flow Cytometry - Anti-Resistin antibody [EPR23334-171] (ab275878)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized U937 (human histiocytic lymphoma monocyte) cells labelling Resistin with ab275878 at 1/500 dilution (0.1µg)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Resistin antibody [EPR23334-171] (ab275878)
    Immunoprecipitation - Anti-Resistin antibody [EPR23334-171] (ab275878)

    Resistin was immunoprecipitated from 0.35 mg U937 (human histiocytic lymphoma monocyte) whole cell lysate with ab275878 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275878 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: U937 whole cell lysate 10 µg

    Lane 2: ab275878 IP in U937 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275878 in U937 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds.

     

  • Western blot - Anti-Resistin antibody [EPR23334-171] (ab275878)
    Western blot - Anti-Resistin antibody [EPR23334-171] (ab275878)
    Anti-Resistin antibody [EPR23334-171] (ab275878) at 1/1000 dilution + Human white fat tissue lysate at 20 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

     

  • Western blot - Anti-Resistin antibody [EPR23334-171] (ab275878)
    Western blot - Anti-Resistin antibody [EPR23334-171] (ab275878)
    Anti-Resistin antibody [EPR23334-171] (ab275878) at 1/1000 dilution + Recombinant human Resistin protein, 20 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 11 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

     

  • Anti-Resistin antibody [EPR23334-171] (ab275878)
    Anti-Resistin antibody [EPR23334-171] (ab275878)

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