Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

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Anti-Resistin antibody [EPR23334-171] (ab275878)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23334-171] to Resistin
Suitable for: WB, Flow Cyt, IP
Reacts with: Human

Product name

Anti-Resistin antibody [EPR23334-171]
See all Resistin primary antibodies
Description

Rabbit monoclonal [EPR23334-171] to Resistin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: U937 whole cell lysate; Human white fat tissue lysate; Recombinant human Resistin protein. Flow Cyt: U937 cells. IP: U937 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23334-171
Isotype

IgG
Research areas

Western blot - Anti-Resistin antibody [EPR23334-171] (ab275878)

All lanes : Anti-Resistin antibody [EPR23334-171] (ab275878) at 1/1000 dilution

Lane 1 : U937 (human histiocytic lymphoma monocyte) whole cell lysate
Lane 2 : THP-1 (human monocytic leukemia monocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID:16532325, 14550293).

Low expression: THP-1 (PMID:16532325 ).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time: 3 minutes.
Flow Cytometry - Anti-Resistin antibody [EPR23334-171] (ab275878)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized U937 (human histiocytic lymphoma monocyte) cells labelling Resistin with ab275878 at 1/500 dilution (0.1µg)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Resistin antibody [EPR23334-171] (ab275878)

Resistin was immunoprecipitated from 0.35 mg U937 (human histiocytic lymphoma monocyte) whole cell lysate with ab275878 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275878 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

Lane 1: U937 whole cell lysate 10 µg

Lane 2: ab275878 IP in U937 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275878 in U937 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 5.5 seconds.
Western blot - Anti-Resistin antibody [EPR23334-171] (ab275878)

Anti-Resistin antibody [EPR23334-171] (ab275878) at 1/1000 dilution + Human white fat tissue lysate at 20 µg

Secondary
VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

Exposure time: 3 minutes.
Western blot - Anti-Resistin antibody [EPR23334-171] (ab275878)

Anti-Resistin antibody [EPR23334-171] (ab275878) at 1/1000 dilution + Recombinant human Resistin protein, 20 ng

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 11 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds.
Anti-Resistin antibody [EPR23334-171] (ab275878)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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