Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)
![Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)](https://www.abcam.com/ps/products/251/ab251302/Images/ab251302-406401-anti-rbmy1a1-antibody-r12508-2-bsa-and-azide-free-western-blot.jpg "Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [R12508(2)] to RBMY1A1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free
See all RBMY1A1 primary antibodies -
Description
Rabbit monoclonal [R12508(2)] to RBMY1A1 - BSA and Azide free -
Host species
Rabbit -
Specificity
Based on sequence analysis, this antibody recognizes 2 isoforms with the predicted MWs of 56KDa and 51KDa respectively. Sequence analysis also showed a 100% homology with RBY1F, RBY1E, RBY1D, RBY1C and RBY1B.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251302 is the carrier-free version of ab200202. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251302 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
R12508(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)All lanes : Anti-RBMY1A1 antibody [R12508(2)] (ab200202) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : Human fetal heart tissue lysate
Lane 4 : Human fetal kidney tissue lysate
Lane 5 : Human fetal spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 51,56 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsThis data was developed using ab200202, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Please note lanes 2-5 represent RBMY1A1 negative lysates.
Based on sequence analysis, ab200202 recognizes 2 isoforms with the predicted MWs of 56KDa and 51KDa respectively. Sequence analysis also showed a 100% homology with RBY1F, RBY1E, RBY1D, RBY1C and RBY1B.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)](https://www.abcam.com/ps/products/251/ab251302/Images/ab251302-2-anti-rbmy1a1-antibody-r12508-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)This data was developed using ab200202, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling RBMY1A1 with ab200202 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining of Human testis tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)](https://www.abcam.com/ps/products/251/ab251302/Images/ab251302-1-anti-rbmy1a1-antibody-r12508-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)This data was developed using ab200202, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue with ab200202 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Please note Human liver tissue represents a RBMY1A1 negative control. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)](https://www.abcam.com/ps/products/251/ab251302/Images/ab251302-4-benefits-of-recombinant-antibodies.png)
Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)