Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rb (phospho T826) antibody [EPR5351] - BSA and Azide free (ab219159)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5351] to Rb (phospho T826) - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Rb (phospho T826) antibody [EPR5351] - BSA and Azide free
    See all Rb primary antibodies

  • Description

    Rabbit monoclonal [EPR5351] to Rb (phospho T826) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects Rb phosphorylated at Threonine 826.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat cell lysate treated with FBS.

  • General notes

    ab219159 is the carrier-free version of ab133446 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab219159 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Rb (phospho T826) antibody [EPR5351] - BSA and Azide free (ab219159)
    Western blot - Anti-Rb (phospho T826) antibody [EPR5351] - BSA and Azide free (ab219159)
    All lanes : Anti-Rb (phospho T826) antibody [EPR5351] (ab133446) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate treated with FBS.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 106 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133446, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-Rb (phospho T826) antibody [EPR5351] - BSA and Azide free (ab219159)
    OI-RD Scanning - Anti-Rb (phospho T826) antibody [EPR5351] - BSA and Azide free (ab219159)
    This data was developed using ab133446, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Rb (phospho T826) antibody [EPR5351] - BSA and Azide free (ab219159)
    Anti-Rb (phospho T826) antibody [EPR5351] - BSA and Azide free (ab219159)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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