Anti-RAP80 antibody (ab70822)
Key features and details
- Rabbit polyclonal to RAP80
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAP80 antibody
See all RAP80 primary antibodies -
Description
Rabbit polyclonal to RAP80 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 600 and 650 of human RAP80 (NP_057374.3).
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Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAP80 antibody (ab70822)All lanes : Anti-RAP80 antibody (ab70822) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 15 µg
Lane 2 : HeLa whole cell lysate at 5 µg
Lane 3 : 293T whole cell lysate at 50 µg
Predicted band size: 80 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 65 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - Anti-RAP80 antibody (ab70822)Detection of Human RAP80 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70822 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
