Anti-CACNB2 antibody [EPR23419-58] - BSA and Azide free (ab275385)
![Anti-CACNB2 antibody [EPR23419-58] - BSA and Azide free (ab275385)](https://www.abcam.com/ps/products/275/ab275385/Images/ab275385-410998-anti-cacnb2-antibody-epr2341958-western-blot-tissue.jpg "Anti-CACNB2 antibody [EPR23419-58] - BSA and Azide free (ab275385)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23419-58] to CACNB2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-CACNB2 antibody [EPR23419-58] - BSA and Azide free
See all CACNB2 primary antibodies -
Description
Rabbit monoclonal [EPR23419-58] to CACNB2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse hippocampus and midbrain tissue lysates; Rat hippocampus and brain tissue lysates.
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General notes
ab275385 is the carrier-free version of ab253193. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275385 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23419-58 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CACNB2 antibody [EPR23419-58] - BSA and Azide free (ab275385)All lanes : Anti-CACNB2 antibody [EPR23419-58] (ab253193) at 1/1000 dilution
Lane 1 : Mouse hippocampus tissue lysate
Lane 2 : Mouse midbrain tissue lysate
Lane 3 : Mouse placenta tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat hippocampus tissue lysate
Lane 6 : Rat placenta tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 73 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?This data was developed using ab253193, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16822546, 24681347).
Negative control: placenta (PMID: 16822546).
Exposure time: Lane 1, 5: 37 seconds Lane 2-3, 4, 6: 114 seconds.
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![Anti-CACNB2 antibody [EPR23419-58] - BSA and Azide free (ab275385)](https://www.abcam.com/ps/products/275/ab275385/Images/ab275385-2-benefits-of-recombinant-antibodies.png)
Anti-CACNB2 antibody [EPR23419-58] - BSA and Azide free (ab275385)
