Anti-RAGE antibody [EPR12205] - BSA and Azide free (ab250494)
![Anti-RAGE antibody [EPR12205] - BSA and Azide free (ab250494)](https://www.abcam.com/ps/products/250/ab250494/Images/ab250494-403497-anti-rage-antibody-epr12205-bsa-and-azide-free-western-blot.jpg "Anti-RAGE antibody [EPR12205] - BSA and Azide free (ab250494)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12205] to RAGE - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-RAGE antibody [EPR12205] - BSA and Azide free
See all RAGE primary antibodies -
Description
Rabbit monoclonal [EPR12205] to RAGE - BSA and Azide free -
Host species
Rabbit -
Specificity
Recent tests in our laboratory showed that the antibody detects the band of interest in tissue lysates, but it did not detect the protein in cell lysate.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250494 is the carrier-free version of ab181369 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250494 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12205 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAGE antibody [EPR12205] - BSA and Azide free (ab250494)All lanes : Anti-RAGE antibody [EPR12205] (ab181369) at 1/1000 dilution
Lane 1 : Mouse lung lysates
Lane 2 : Mouse brain lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse heart lysates
Lane 5 : Mouse liver lysates
Lane 6 : Mouse spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg
Predicted band size: 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsThis data was developed using ab181369, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The expression profile and molecular mass observed is consistent with what has been described in the literature (PMID: 16315007; 18355449; 18245812).
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![Western blot - Anti-RAGE antibody [EPR12205] - BSA and Azide free (ab250494)](https://www.abcam.com/ps/products/250/ab250494/Images/ab250494-398739-anti-rage-antibody-epr12205-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-RAGE antibody [EPR12205] - BSA and Azide free (ab250494) -
![Anti-RAGE antibody [EPR12205] - BSA and Azide free (ab250494)](https://www.abcam.com/ps/products/250/ab250494/Images/ab250494-3-benefits-of-recombinant-antibodies.png)
Anti-RAGE antibody [EPR12205] - BSA and Azide free (ab250494)
