Anti-Raf1 antibody (ab137435)
Key features and details
- Rabbit polyclonal to Raf1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Raf1 antibody
See all Raf1 primary antibodies -
Description
Rabbit polyclonal to Raf1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseHumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Raf1 (internal sequence).
Database link: P04049 -
Positive control
- WB: 200nM PMA treated (+) HeLa whole cell extracts starved for 24 hours. IHC-P: mouse muscle tissue, human endometrial carcinoma tissue, A549 xenograft. ICC/IF: A549 cells, A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Raf1 antibody (ab137435)All lanes : Anti-Raf1 antibody (ab137435) at 1/1000 dilution
Lane 1 : Untreated (–) HeLa whole cell extracts starved for 24 hours
Lane 2 : 200nM PMA treated (+) HeLa whole cell extracts starved for 24 hours
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 73 kDa
Additional bands at: 150 kDa (possible dimer)Raf1 protein may migrate to higher size when phosphorylated and appears as doublet due to different phosphorylation status.
7.5% SDS gel.
Running condition: 80V, 15min; 140V, 40 min.
Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60 min.
Primary antibody incubation: 4°C overnight.
Washing condition: 5 ml TBST, 4 x 5 min.
Exposure: Trident plus Western HRP Substrate.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 antibody (ab137435)Immunohistochemical analysis of paraffin-embedded mouse muscle tissue labeling Raf1 with ab137435 at 1/500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. Nuclear staining is observed.
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Immunocytochemistry/ Immunofluorescence - Anti-Raf1 antibody (ab137435) This image is courtesy of an AbReview submitted by Dimitra Kalamida.Immunocytochemistry/Immunofluorescence analysis A549 cells labeling Raf1 with ab137435 at 1/200 dilution. The cells were fixed with Paraformaldehyde and permeabilized with 0.1% v/v Triton X-100 pH 7.4. CF568 Donkey anti-rabbit IgG(H+L) polyclonal was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 antibody (ab137435)Immunohistochemical analysis of formalin-fixed paraffin-embedded human endometrial carcinoma tissue section labeling Raf1 with ab137435 at 1/500.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 antibody (ab137435)Immunohistochemical analysis of parafin-embedded A549 xenograft labelling Raf1 with ab137435 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-Raf1 antibody (ab137435)Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labelling Raf1 with ab137435 at 1/200 dilution. The image in the lower panel is merged with a DNA probe.
