Anti-MAK antibody [OTI1G2] (ab279383)
![Anti-MAK antibody [OTI1G2] (ab279383)](https://www.abcam.com/ps/products/279/ab279383/Images/ab279383-6-AntiMAK-antibody-OTI1G2-immunohistochemistry-Carcinoma-prostate-tissue-human.jpg "Anti-MAK antibody [OTI1G2] (ab279383)")
Key features and details
- Mouse monoclonal [OTI1G2] to MAK
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-MAK antibody [OTI1G2] -
Description
Mouse monoclonal [OTI1G2] to MAK -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human MAK aa 291-623. NP_005897
Database link: P20794 -
Positive control
- WB: HEK-293T, HeLa, HepG2 cell. IHC-P: Carcinoma of human prostate tissue, thyroid carcinoma tissue, pancreas tissue, liver tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
OTI1G2 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)
Paraffin embedded carcinoma of human prostate tissue stained for MAK using ab279383 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)](https://www.abcam.com/ps/products/279/ab279383/Images/ab279383-5-AntiMAK-antibody-OTI1G2-immunohistochemistry-Carcinoma-thyroid-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)Paraffin embedded carcinoma of human thyroid tissue stained for MAK using ab279383 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)](https://www.abcam.com/ps/products/279/ab279383/Images/ab279383-4-AntiMAK-antibody-OTI1G2-immunohistochemistry-pancreas-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)Paraffin embedded human pancreas tissue stained for MAK using ab279383 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)](https://www.abcam.com/ps/products/279/ab279383/Images/ab279383-3-AntiMAK-antibody-OTI1G2-immunohistochemistry-liver-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (ab279383)Paraffin embedded human liver tissue stained for MAK using ab279383 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.
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![Western blot - Anti-MAK antibody [OTI1G2] (ab279383)](https://www.abcam.com/ps/products/279/ab279383/Images/ab279383-2-AntiMAK-antibody-OTI1G2-westernblot-293T-HeLa-HepG2-cells.jpg)
Western blot - Anti-MAK antibody [OTI1G2] (ab279383)All lanes : Anti-MAK antibody [OTI1G2] (ab279383) at 1/500 dilution
Lane 1 : HEK-293T cell extract
Lane 2 : HeLa cell extract
Lane 3 : HepG2 cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 71 kDa
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![Western blot - Anti-MAK antibody [OTI1G2] (ab279383)](https://www.abcam.com/ps/products/279/ab279383/Images/ab279383-1-AntiMAK-antibody-OTI1G2-westernblot-HEKP293T-cells.jpg)
Western blot - Anti-MAK antibody [OTI1G2] (ab279383)All lanes : Anti-MAK antibody [OTI1G2] (ab279383) at 1/2000 dilution
Lane 1 : HEK-293T cells transfected with the pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY MAK
Lysates/proteins at 5 µg per lane.
Predicted band size: 71 kDa
