Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases

Human PLK2 knockout HCT116 cell lysate (ab263304)

Human PLK2 knockout HCT116 cell lysate (ab263304)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

You may also be interested in

Product image
Anti-WNK2 antibody (ab192397)
Product image
Anti-Serine/threonine-protein kinase 4/MST-1 antibody (ab264127)
Product image
Anti-ULK1 (phospho S638) antibody (ab229755)
Product image
Human VRK2 knockout HeLa cell line (ab265881)

Overview

  • Product name

    Human PLK2 knockout HCT116 cell lysate
    See all PLK2 kits
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HCT116
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon1.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab263748 - Human PLK2 knockout HCT116 cell lysate 1 x 100µg
    ab255555 - Human wild-type HCT116 cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
  • Cell type

    epithelial
  • Disease

    Carcinoma
  • STR Analysis

    Amelogenin X D5S818: 10, 11 D13S317: 10, 12 D7S820: 11, 12 D16S539: 11, 13 vWA: 17, 22 TH01: 8,9 TPOX: 8, 9 CSF1PO: 7, 10

Target

  • Function

    May play a role in the division of at least some cell types, such as fibroblasts, and could function in embryogenesis, wound healing or neoplasia.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession Q9NYY3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • hPlk2
    • hSNK
    • PLK-2
    • Plk2
    • PLK2_HUMAN
    • Polo like kinase 2
    • Polo-like kinase 2
    • Serine/threonine protein kinase PLK2
    • Serine/threonine protein kinase SNK
    • Serine/threonine-protein kinase PLK2
    • Serine/threonine-protein kinase SNK
    • Serum Induced Kinase
    • Serum-inducible kinase
    • SNK
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab263748 - Human PLK2 knockout HCT116 cell lysate 1 x 100µg
    ab255555 - Human wild-type HCT116 cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
  • Cell type

    epithelial
  • Disease

    Carcinoma
  • STR Analysis

    Amelogenin X D5S818: 10, 11 D13S317: 10, 12 D7S820: 11, 12 D16S539: 11, 13 vWA: 17, 22 TH01: 8,9 TPOX: 8, 9 CSF1PO: 7, 10

Images

  • Sanger Sequencing - Human PLK2 knockout HCT116 cell lysate (ab263304)
    Sanger Sequencing - Human PLK2 knockout HCT116 cell lysate (ab263304)
    Homozygous: 2 bp deletion in exon1

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free (ab170969)

  •  
  • Product image

    Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (ab250878)

  •  
  • Product image

    Anti-MCP1 antibody [EPR21025] - Low endotoxin, Azide free (ab246795)

  •  
  • Product image

    FGR overexpression 293T lysate (whole cell) (ab94154)

  •  
  • Recombinant human TARC/CCL17 protein (ab9817)

  •  
  • Product image

    Recombinant Human MAFF protein (denatured) (ab156322)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.