Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RABL3 antibody [EPR16709] - BSA and Azide free (ab236018)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16709] to RABL3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-RABL3 antibody [EPR16709] - BSA and Azide free
    See all RABL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR16709] to RABL3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human renal adenocarcinoma tissue.

  • General notes

    ab236018 is the carrier-free version of ab196024.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - BSA and Azide free (ab236018)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - BSA and Azide free (ab236018)

    Immunohistochemical analysis of paraffin-embedded, rat liver tissue labeling RABL3 with ab196024 at a 1/100 dilution. Counter stained with hematoxylin. As secondary antibody goat anti-rabbit IgG H&L (HRP) ab97051 was used at a 1/500. In blue DAPI staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196024).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RABL3 antibody [EPR16709] - BSA and Azide free (ab236018)
    Immunocytochemistry/ Immunofluorescence - Anti-RABL3 antibody [EPR16709] - BSA and Azide free (ab236018)

    Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labeling RABL3 with ab196024 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/500. In blue DAPI staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196024).

  • Flow Cytometry - Anti-RABL3 antibody [EPR16709] - BSA and Azide free (ab236018)
    Flow Cytometry - Anti-RABL3 antibody [EPR16709] - BSA and Azide free (ab236018)

    Flow cytometry analysis of paraformaldehyde-fixed MCF7 cells labeling RABL3 with ab196024 at a 1/100 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, isotype control rabbit IgG (black) and unlabeled control cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196024).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - BSA and Azide free (ab236018)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - BSA and Azide free (ab236018)

    Immunohistochemical analysis of paraffin-embedded, human renal adenocarcinoma tissue labeling RABL3 with ab196024 at a 1/100 dilution. Counter stained with hematoxylin. As secondary antibody goat anti-rabbit IgG H&L (HRP) ab97051 was used at a 1/500. In blue DAPI staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196024).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-RABL3 antibody [EPR16709] - BSA and Azide free (ab236018)
    Anti-RABL3 antibody [EPR16709] - BSA and Azide free (ab236018)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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