Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free (ab247546)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3500] to GTPBP4/NOG1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free
    See all GTPBP4/NOG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3500] to GTPBP4/NOG1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247546 is the carrier-free version of ab92342 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247546 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as GTPBP4

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free (ab247546)
    Western blot - Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free (ab247546)
    All lanes : Anti-GTPBP4/NOG1 antibody [EPR3500] (ab92342) at 1/2000 dilution

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Human fetal brain tissue lysate
    Lane 3 : Human placenta tissue lysate
    Lane 4 : Human testis tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 74 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab92342, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free (ab247546)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free (ab247546)
    This data was developed using ab92342, the same antibody clone in a different buffer formulation.ab92342 at 1/250 dilution staining GTPBP4/NOG1 in Human kidney by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free (ab247546)
    Anti-GTPBP4/NOG1 antibody [EPR3500] - BSA and Azide free (ab247546)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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