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Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16709] to RABL3 - C-terminal
  • Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-RABL3 antibody [EPR16709] - C-terminal
    See all RABL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR16709] to RABL3 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF7, K562 and 293 cell lysates. Human renal adenocarcinoma and rat liver tissues. A549 and MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)
    Western blot - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)
    Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024) at 1/10000 dilution + MCF7 cell lysate at 20 µg

    Secondary
    Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

  • Western blot - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)
    Western blot - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)
    All lanes : Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)

    Immunohistochemical analysis of paraffin-embedded, human renal adenocarcinoma tissue labeling RABL3 with ab196024 at a 1/100 dilution. Counter stained with hematoxylin. As secondary antibody goat anti-rabbit IgG H&L (HRP) ab97051 was used at a 1/500. In blue DAPI staining.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)

    Immunohistochemical analysis of paraffin-embedded, rat liver tissue labeling RABL3 with ab196024 at a 1/100 dilution. Counter stained with hematoxylin. As secondary antibody goat anti-rabbit IgG H&L (HRP) ab97051 was used at a 1/500. In blue DAPI staining.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)
    Immunocytochemistry/ Immunofluorescence - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)

    Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labeling RABL3 with ab196024 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/500. In blue DAPI staining.

  • Flow Cytometry - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)
    Flow Cytometry - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)

    Flow cytometry analysis of paraformaldehyde-fixed MCF7 cells labeling RABL3 with ab196024 at a 1/100 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, isotype control rabbit IgG (black) and unlabeled control cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)
    Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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