Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB21 antibody (ab88762)

Key features and details

  • Rabbit polyclonal to RAB21
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RAB21 antibody
    See all RAB21 primary antibodies

  • Description

    Rabbit polyclonal to RAB21

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human RAB21 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab89377)

  • Positive control

    • This antibody gave a positive signal in Human liver tissue lysate and in the following whole cell lysates: HepG2; HeLa; A431; Jurkat.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab88762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Cow
Dog
Zebrafish
Application Abreviews Notes
WB (1)
Notes
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration (By similarity). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis.

    • Tissue specificity

      Widely expressed. In jejunal tissue, predominantly expressed in the apical region of the epithelial cell layer of the villi, weak expression, if any, in the crypt epithelium. Capillary endothelium and some cell types in the lamina propria also show expression.

    • Sequence similarities

      Belongs to the small GTPase superfamily. Rab family.

    • Cellular localization

      Endoplasmic reticulum membrane. Golgi apparatus membrane. Early endosome membrane. Cytoplasmic vesicle membrane. Cleavage furrow. In nonpolarized epithelial Caco-2 cells, found in the endoplasmic reticulum; in polarized cells, observed in vesicles in the apical cytoplasm. During mitosis, in mid-telophase, localized in the ingressing cleavage furrow. In late telophase, detected at the opposite poles of the daughter cells, in vesicles at the base of lamellipodia formed by the separating daughter cells.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • KIAA0118 antibody
      • RAB 21 antibody
      • RAB21 antibody
      • RAB21, member RAS oncogene family antibody
      • RAB21_HUMAN antibody
      • Ras-related protein Rab-21 antibody
      see all

    Images

    • Western blot - Anti-RAB21 antibody (ab88762)
      Western blot - Anti-RAB21 antibody (ab88762)
      All lanes : Anti-RAB21 antibody (ab88762) at 1 µg/ml

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 5 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 24 kDa
      Observed band size: 26 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 12.5 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes

    References (0)

    Publishing research using ab88762? Please let us know so that we can cite the reference in this datasheet.

    ab88762 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-RAB21 antibody (ab88762)
      Western blot - Anti-RAB21 antibody (ab88762)
      All lanes : Anti-RAB21 antibody (ab88762) at 1 µg/ml

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 5 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 24 kDa
      Observed band size: 26 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 12.5 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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