Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB21 antibody (ab79713)

Key features and details

  • Rabbit polyclonal to RAB21
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RAB21 antibody
    See all RAB21 primary antibodies

  • Description

    Rabbit polyclonal to RAB21

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human RAB21 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab89375)

  • Positive control

    • This antibody gave a positive signal in Human liver tissue lysate and in the following whole cell lysates: HepG2; HeLa; A431; Jurkat.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab79713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Sheep
Cow
Dog
Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).

Target

  • Function

    Regulates integrin internalization and recycling, but does not influence the traffic of endosomally translocated receptors in general. As a result, may regulate cell adhesion and migration (By similarity). During the mitosis of adherent cells, controls the endosomal trafficking of integrins which is required for the successful completion of cytokinesis.

  • Tissue specificity

    Widely expressed. In jejunal tissue, predominantly expressed in the apical region of the epithelial cell layer of the villi, weak expression, if any, in the crypt epithelium. Capillary endothelium and some cell types in the lamina propria also show expression.

  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.

  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Early endosome membrane. Cytoplasmic vesicle membrane. Cleavage furrow. In nonpolarized epithelial Caco-2 cells, found in the endoplasmic reticulum; in polarized cells, observed in vesicles in the apical cytoplasm. During mitosis, in mid-telophase, localized in the ingressing cleavage furrow. In late telophase, detected at the opposite poles of the daughter cells, in vesicles at the base of lamellipodia formed by the separating daughter cells.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • KIAA0118 antibody
    • RAB 21 antibody
    • RAB21 antibody
    • RAB21, member RAS oncogene family antibody
    • RAB21_HUMAN antibody
    • Ras-related protein Rab-21 antibody
    see all

Images

  • Western blot - Anti-RAB21 antibody (ab79713)
    Western blot - Anti-RAB21 antibody (ab79713)
    All lanes : Anti-RAB21 antibody (ab79713) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 12.5 kDa, 146 kDa, 38 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

References (0)

Publishing research using ab79713? Please let us know so that we can cite the reference in this datasheet.

ab79713 has not yet been referenced specifically in any publications.

Images

  • Western blot - Anti-RAB21 antibody (ab79713)
    Western blot - Anti-RAB21 antibody (ab79713)
    All lanes : Anti-RAB21 antibody (ab79713) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 12.5 kDa, 146 kDa, 38 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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