Anti-RAB14 antibody (ab40938)
Key features and details
- Rabbit polyclonal to RAB14
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RAB14 antibody -
Description
Rabbit polyclonal to RAB14 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig, Orangutan -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RAB14.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentrationConcentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab40938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 24 kDa). Target
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Function
May be involved in vesicular trafficking and neurotransmitter release. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 417119 Chicken
- Entrez Gene: 51552 Human
- Entrez Gene: 68365 Mouse
- Entrez Gene: 595112 Pig
- Entrez Gene: 94197 Rat
- Omim: 612673 Human
- SwissProt: P61106 Human
- SwissProt: Q91V41 Mouse
see all -
Alternative names
- bA165P4.3 antibody
- F protein binding protein 1 antibody
- FBP antibody
see all
Images
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All lanes : Anti-RAB14 antibody (ab40938) at 1 mg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
Performed under non-reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 8 minutes -
All lanes : Anti-RAB14 antibody (ab40938) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Mouse Tissue Lysate
Lane 3 : Brain (Rat) Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
The RAB14 protein has several Ser/Thr residues which may be phosphorylated (SwissProt data). This could cause the doublet observed at the expected molecular weight.
Datasheets and documents
References (7)
ab40938 has been referenced in 7 publications.
- Hoshijima M et al. Roles of Interaction between CCN2 and Rab14 in Aggrecan Production by Chondrocytes. Int J Mol Sci 21:N/A (2020). PubMed: 32316324
- Capmany A et al. Akt/AS160 Signaling Pathway Inhibition Impairs Infection by Decreasing Rab14-Controlled Sphingolipids Delivery to Chlamydial Inclusions. Front Microbiol 10:666 (2019). PubMed: 31001235
- Xiong X et al. Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins. Biol Open 8:N/A (2019). PubMed: 31142470
- Yu J et al. Rab14 Suppression Mediated by MiR-320a Inhibits Cell Proliferation, Migration and Invasion in Breast Cancer. J Cancer 7:2317-2326 (2016). WB . PubMed: 27994670
- Capmany A & Damiani MT Chlamydia trachomatis intercepts Golgi-derived sphingolipids through a Rab14-mediated transport required for bacterial development and replication. PLoS One 5:e14084 (2010). ICC/IF ; Human . PubMed: 21124879
- Comellas AP et al. Insulin regulates alveolar epithelial function by inducing Na+/K+-ATPase translocation to the plasma membrane in a process mediated by the action of Akt. J Cell Sci 123:1343-51 (2010). WB ; Human . PubMed: 20332111
- Kelly EE et al. Class I Rab11-family interacting proteins are binding targets for the Rab14 GTPase. Biol Cell 102:51-62 (2009). PubMed: 19702578
Images
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All lanes : Anti-RAB14 antibody (ab40938) at 1 mg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
Performed under non-reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 8 minutes
-
All lanes : Anti-RAB14 antibody (ab40938) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Mouse Tissue Lysate
Lane 3 : Brain (Rat) Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
The RAB14 protein has several Ser/Thr residues which may be phosphorylated (SwissProt data). This could cause the doublet observed at the expected molecular weight.