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Signal Transduction Protein Trafficking Organelle Proteins

Anti-RAB14 antibody (ab40938)

Price and availability

288 134 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-RAB14 antibody (ab40938)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to RAB14
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RAB14 antibody
  • Description

    Rabbit polyclonal to RAB14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RAB14.

    Read Abcam's proprietary immunogen policy (Peptide available as ab41804.)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human RAB14 protein (ab90105)

Applications

Our Abpromise guarantee covers the use of ab40938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 24 kDa).

Target

  • Function

    May be involved in vesicular trafficking and neurotransmitter release.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P61106 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 417119 Chicken
    • Entrez Gene: 51552 Human
    • Entrez Gene: 68365 Mouse
    • Entrez Gene: 595112 Pig
    • Entrez Gene: 94197 Rat
    • Omim: 612673 Human
    • SwissProt: P61106 Human
    • SwissProt: Q91V41 Mouse
    • SwissProt: P61107 Rat
    • Unigene: 371563 Human
    • Unigene: 713585 Human
    • Unigene: 198264 Mouse
    • Unigene: 6008 Rat
    see all
  • Alternative names

    • bA165P4.3 antibody
    • F protein binding protein 1 antibody
    • FBP antibody
    • GTPase Rab14 antibody
    • RAB 14 antibody
    • RAB14 antibody
    • RAB14 member RAS oncogene family antibody
    • RAB14_HUMAN antibody
    • Ras related protein Rab 14 antibody
    • Ras-related protein Rab-14 antibody
    • RP11 165P4.4 antibody
    • Small GTP binding protein RAB14 antibody
    see all

Images

  • Western blot - Anti-RAB14 antibody (ab40938)
    Western blot - Anti-RAB14 antibody (ab40938)
    All lanes : Anti-RAB14 antibody (ab40938) at 1 mg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution

    Performed under non-reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 24 kDa


    Exposure time: 8 minutes
  • Western blot - Anti-RAB14 antibody (ab40938)
    Western blot - Anti-RAB14 antibody (ab40938)
    All lanes : Anti-RAB14 antibody (ab40938) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Mouse Tissue Lysate
    Lane 3 : Brain (Rat) Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?



    The RAB14 protein has several Ser/Thr residues which may be phosphorylated (SwissProt data). This could cause the doublet observed at the expected molecular weight.

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (7)

    Publishing research using ab40938? Please let us know so that we can cite the reference in this datasheet.

    ab40938 has been referenced in 7 publications.

    • Hoshijima M  et al. Roles of Interaction between CCN2 and Rab14 in Aggrecan Production by Chondrocytes. Int J Mol Sci 21:N/A (2020). PubMed: 32316324
    • Capmany A  et al. Akt/AS160 Signaling Pathway Inhibition Impairs Infection by Decreasing Rab14-Controlled Sphingolipids Delivery to Chlamydial Inclusions. Front Microbiol 10:666 (2019). PubMed: 31001235
    • Xiong X  et al. Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins. Biol Open 8:N/A (2019). PubMed: 31142470
    • Yu J  et al. Rab14 Suppression Mediated by MiR-320a Inhibits Cell Proliferation, Migration and Invasion in Breast Cancer. J Cancer 7:2317-2326 (2016). WB . PubMed: 27994670
    • Capmany A & Damiani MT Chlamydia trachomatis intercepts Golgi-derived sphingolipids through a Rab14-mediated transport required for bacterial development and replication. PLoS One 5:e14084 (2010). ICC/IF ; Human . PubMed: 21124879
    • Comellas AP  et al. Insulin regulates alveolar epithelial function by inducing Na+/K+-ATPase translocation to the plasma membrane in a process mediated by the action of Akt. J Cell Sci 123:1343-51 (2010). WB ; Human . PubMed: 20332111
    • Kelly EE  et al. Class I Rab11-family interacting proteins are binding targets for the Rab14 GTPase. Biol Cell 102:51-62 (2009). PubMed: 19702578

    Images

    • Western blot - Anti-RAB14 antibody (ab40938)
      Western blot - Anti-RAB14 antibody (ab40938)
      All lanes : Anti-RAB14 antibody (ab40938) at 1 mg/ml

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : Brain (Mouse) Tissue Lysate
      Lane 3 : Brain (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution

      Performed under non-reducing conditions.

      Predicted band size: 24 kDa
      Observed band size: 24 kDa


      Exposure time: 8 minutes
    • Western blot - Anti-RAB14 antibody (ab40938)
      Western blot - Anti-RAB14 antibody (ab40938)
      All lanes : Anti-RAB14 antibody (ab40938) at 1 µg/ml

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : Brain (Mouse) Mouse Tissue Lysate
      Lane 3 : Brain (Rat) Rat Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 24 kDa
      Observed band size: 23 kDa
      why is the actual band size different from the predicted?



      The RAB14 protein has several Ser/Thr residues which may be phosphorylated (SwissProt data). This could cause the doublet observed at the expected molecular weight.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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