Anti-Rab-6 antibody (ab95954)
Key features and details
- Rabbit polyclonal to Rab-6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Rab-6 antibody -
Description
Rabbit polyclonal to Rab-6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Rab-6.
Database link: P20340 -
Positive control
- WB: Transfected HeLa, A549, H1299, HCT 116 cells, mouse and rat brain tissue lysate; IHC-P: MDA-MB 468 xenograft tissue; IF: A549 cells.
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General notes
We have not tested the cross reactivity to RAB6B. ab95954 is raised against a full length RAB6A recombinant protein and may cross-react with RAB6B.
Protein previously labeled as RAB6A.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Rab-6 antibody (ab95954) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 24 kDa12 % SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded MDA-MB 468 xenograft tissue staining Rab-6 with ab95954 at 1:500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunofluorescence analysis of 4% paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cell labling Rab-6 protein at Golgi apparatus with ab95954 at 1:500 dilution. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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All lanes : Anti-Rab-6 antibody (ab95954) at 1/500 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Rab-6 knockout HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 24 kDa12% SDS-PAGE
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Anti-Rab-6 antibody (ab95954) at 1/1000 dilution + Rat brain tissue lysate at 50 µg
Predicted band size: 24 kDa12 % SDS-PAGE
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Anti-Rab-6 antibody (ab95954) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg
Predicted band size: 24 kDa12 % SDS-PAGE