Anti-RHEB antibody (ab109914)
Key features and details
- Rabbit polyclonal to RHEB
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RHEB antibody
See all RHEB primary antibodies -
Description
Rabbit polyclonal to RHEB -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: Raji; SHSY5Y; EGF treated A431; Jurkat.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications RatRabbitHorseCowPigChimpanzeeMacaque monkeyGorillaApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).Target
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Function
Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. -
Tissue specificity
Ubiquitous. Highest levels observed in skeletal and cardiac muscle. -
Sequence similarities
Belongs to the small GTPase superfamily. Rheb family. -
Post-translational
modificationsFarnesylation is important for efficiently activating mTORC1-mediated signaling. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 528204 Cow
- Entrez Gene: 6009 Human
- Entrez Gene: 26954 Rat
- Omim: 601293 Human
- SwissProt: Q56JV3 Cow
- SwissProt: Q15382 Human
- SwissProt: Q62639 Rat
- Unigene: 283521 Human
see all -
Alternative names
- Ras homolog enriched in brain 2, formerly antibody
- GTP binding protein Rheb antibody
- GTP-binding protein Rheb antibody
see all
Images
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Western blot - Anti-RHEB antibody (ab109914)All lanes : Anti-RHEB antibody (ab109914) at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : EGF-Stimulated A431 Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa, 49 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109914 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab109914 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-RHEB antibody (ab109914)All lanes : Anti-RHEB antibody (ab109914) at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : EGF-Stimulated A431 Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa, 49 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109914 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
