Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22504-36] to PSGL-1 - BSA and Azide free
  • Suitable for: IP, WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free
    See all PSGL-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22504-36] to PSGL-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Jurkat cells. IHC-P: Human tonsil, cerebrum and colon tissue. Flow: Human peripheral blood mononuclear cells. IP: Jurkat whole cell lysate.

  • General notes

    Ab255290 is the carrier-free version of ab227836. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255290 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PSGL-1 with ab227836 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human tonsil (PMID: 19594630, 16567389). The section was incubated with ab227836 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227836).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PSGL-1 with ab227836 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on infiltrated leukocytes of human colon (PMID: 19594630). The section was incubated with ab227836 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227836).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling PSGL-1 with ab227836 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on microglial cells of human cerebrum is observed. The section was incubated with ab227836 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227836).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
    Immunocytochemistry/ Immunofluorescence - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia T lymphocyte) cells stained for PSGL-1 using ab227836 (Green) at 1/50 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Confocal image showing membranous staining in Jurkat cells. The nuclear counterstain was DAPI (Blue). Tubulin was stained using the Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227836).

  • Flow Cytometry - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
    Flow Cytometry - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cell line labeling PSGL-1 using ab227836 at 1/500 dilution (Right). Cells were stained with rabbit monoclonal IgG (ab172730) (Left) or ab227836 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647. Gated on viable cells. The secondary antibody was a Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/2000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227836).

  • Immunoprecipitation - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
    Immunoprecipitation - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)

    PSGL-1 was immunoprecipitated form 0.35 mg of Jurkat (human T cell leukemia T lymphocyte) whole cell lysate using ab227836 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab227836 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary at a 1/1000 dilution.

    Lane 1: Jurkat whole cell lysate µg (input).
    Lane 2: ab227836 IP in jurkat whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227836 in Jurkat whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227836).

    The expression profile observed is consistent with what has been described in the literature (PMID: 9660879; PMID: 28515724). PSGL-1(P-selectin is the P-selectin glycoprotein ligand-1) is a 125KD glycoprtein and the 250KD band represents the dimer.

  • Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
    Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)

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