Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24509-70] to Protein S - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Protein S antibody [EPR24509-70] - BSA and Azide free
    See all Protein S primary antibodies

  • Description

    Rabbit monoclonal [EPR24509-70] to Protein S - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt (Intra),ICC or IHC-Fr

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Huh7, Human liver, lung, stomach, plasma lysates, HepG2. IHC-P: Human testis and colon. IP: Human liver cell.

  • General notes

    ab280894 is the carrier-free version of ab280885. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280894 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)
    Western blot - Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)
    All lanes : Anti-Protein S antibody [EPR24509-70] (ab280885) at 1/1000 dilution

    Lane 1 : Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate
    Lane 2 : Human liver tissue lysate
    Lane 3 : Human lung tissue lysate 20
    Lane 4 : Human stomach tissue lysate
    Lane 5 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate
    Lane 6 : HaCaT (human skin keratinocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 75 kDa
    Observed band size: 85,75,65 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab280885, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:28118606, 27447084).

    Negative control: HaCaT (PMID:28118606).

    The blot of lane 1 was developed using a higher sensitivity ECL substrate.

    Exposure time: Lane 1: 92 seconds Lane 2: 48 seconds Lane 3: 26 seconds Lane 4-6: 3 minutes

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)

    This data was developed using ab280885, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human testis tissue labelling Protein S with ab280885 at 1/1000 dilution (1.603 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the leydig cells in human testis (PMID: 7945211). The section was incubated with ab280885 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)
    Immunoprecipitation - Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)

    This data was developed using ab280885, the same antibody clone in a different buffer formulation.

    Protein S was immunoprecipitated from 0.35 mg of Human liver tissue lysate with ab280885 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280885 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human liver tissue lysate 10 ug

    Lane 2: ab280885 IP in Human liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280885 in human liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

  • Western blot - Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)
    Western blot - Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)
    Anti-Protein S antibody [EPR24509-70] (ab280885) at 1/2000 dilution + Human plasma at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 75 kDa
    Observed band size: 85,75 kDa why is the actual band size different from the predicted?



    This data was developed using ab280885, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:28118606, 27447084).

    Exposure time: 15 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)

    This data was developed using ab280885, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Protein S with ab280885 at 1/1000 dilution (1.603 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the plasma in human colon. The section was incubated with ab280885 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)

    This data was developed using ab280885, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling Protein S with ab280885 at 1/1000 dilution (1.603 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on human pancreas (PMID:7822769). The section was incubated with ab280885 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)
    Anti-Protein S antibody [EPR24509-70] - BSA and Azide free (ab280894)

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