Anti-uPA Receptor/U-PAR antibody (ab103791)
Key features and details
- Rabbit polyclonal to uPA Receptor/U-PAR
- Suitable for: WB, ICC
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-uPA Receptor/U-PAR antibody
See all uPA Receptor/U-PAR primary antibodies -
Description
Rabbit polyclonal to uPA Receptor/U-PAR -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human uPA Receptor/U-PAR aa 290-304 (C terminal).
Sequence:KSGCNHPDLDVQYRS
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-uPA Receptor/U-PAR antibody (ab103791) at 1 µg/ml
Lane 1 : Human placenta tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat thymus tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : MCF7 whole cell lysate
Lane 6 : SMMC whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : Raji whole cell lysate
Lane 9 : Colo320 whole cell lysate
Predicted band size: 37 kDa
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ab103791, at 1µg/ml, staining uPA Receptor/U-PAR in MCF7 cells by Immunocytochemistry.
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MDA-MB-231 cells were incubated at 37°C for 24h with vehicle control (0 µM) and varied concentrations of SB 203580 hydrochloride (ab120235). Decreased expression of uPA receptor/U-PAR in MDA-MB-231 cells correlates with an increase in SB 203580 hydrochloride concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab103791 at 1 µg/ml and ab1801 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.