Anti-Prostate Specific Antigen antibody [SP317] (ab224799)
![Anti-Prostate Specific Antigen antibody [SP317] (ab224799)](https://www.abcam.com/ps/products/224/ab224799/Images/ab224799-3-AntiProstate-Specific-Antigen-antibody-SP317-IHC-human-prostate.jpg "Anti-Prostate Specific Antigen antibody [SP317] (ab224799)")
Key features and details
- Rabbit monoclonal [SP317] to Prostate Specific Antigen
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Prostate Specific Antigen antibody [SP317]
See all Prostate Specific Antigen primary antibodies -
Description
Rabbit monoclonal [SP317] to Prostate Specific Antigen -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Prostate Specific Antigen (internal sequence). The exact sequence is proprietary.
Database link: P07288 -
Positive control
- WB: LNCaP cell lysate. IHC-P: Human prostate tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP317 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] (ab224799)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate tissue sections labeling Prostate Specific Antigen with ab224799 at 1/100 dilution (3.83 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on the human prostate, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224799 for 10 mins at room temperature. -
![Western blot - Anti-Prostate Specific Antigen antibody [SP317] (ab224799)](https://www.abcam.com/ps/products/224/ab224799/Images/ab224799-286008-anti-prostate-specific-antigen-antibody-sp317-western-blot.jpg)
Western blot - Anti-Prostate Specific Antigen antibody [SP317] (ab224799)Anti-Prostate Specific Antigen antibody [SP317] (ab224799) at 1/100 dilution + LNCaP (human prostate cancer cell line) cell lysate
Predicted band size: 29 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] (ab224799)](https://www.abcam.com/ps/products/224/ab224799/Images/ab224799-286009-anti-prostate-specific-antigen-antibody-sp317-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [SP317] (ab224799)Formalin-fixed, paraffin-embedded human prostate tissue stained for Prostate Specific Antigen using ab224799 at 1/100 dilution in immunohistochemical analysis.
