Anti-Profilin antibody (ab225944)
Key features and details
- Rabbit polyclonal to Profilin
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Profilin antibody
See all Profilin primary antibodies -
Description
Rabbit polyclonal to Profilin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein (His-tag) corresponding to Profilin aa 1 to the C-terminus. (Mercurialis annua; 6xHis-SUMO tag on N-terminus).
Database link: O49894 -
Positive control
- WB: Recombinant Mercurialis annua Profilin protein with 6xHis-SUMO tag on N-terminus.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-Profilin antibody (ab225944)All lanes : Anti-Profilin antibody (ab225944) at 1/500 dilution
Lane 1 : Recombinant Mercurialis annua Profilin protein with 6xHis-SUMO tag on N-terminus at 0.08 µg
Lane 2 : Recombinant Mercurialis annua Profilin protein with 6xHis-SUMO tag on N-terminus at 0.04 µg
Lane 3 : Recombinant Mercurialis annua Profilin protein with 6xHis-SUMO tag on N-terminus at 0.02 µg
Lane 4 : Recombinant Mercurialis annua Profilin protein with 6xHis-SUMO tag on N-terminus at 0.01 µg
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?Predicted MW of recombinant protein: 31 kDa
