Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-pro-EGF antibody [EPR14622] (ab206106)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14622] to pro-EGF
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-pro-EGF antibody [EPR14622]
    See all pro-EGF primary antibodies

  • Description

    Rabbit monoclonal [EPR14622] to pro-EGF

  • Host species

    Rabbit

  • Specificity

    Recognises the Pro-epidermal growth factor chain.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human EGF recombinant protein; Human milk sample; Human urine sample. IP: Human milk sample

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106)
    Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106)
    All lanes : Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution

    Lane 1 : Recombinant Human Pro-EGF Protein (aa 21-1023) with His-Tag
    Lane 2 : Recombinant Human EGF recombinant protein (aa971-1023)

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 134 kDa



    Blocking and Diluting buffer and concentration:5% NFDM/TBST

    Exposure time:1 second

  • Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106)
    Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106)
    Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/5000 dilution + Human EGF recombinant protein at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 134 kDa
    Observed band size: 160 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human EGF full length protein contains aa21-1023 with His-Tag® at the C-Terminus.

  • Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106)
    Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106)
    Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution + Human milk sample at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 134 kDa
    Observed band size: 160 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106)
    Western blot - Anti-pro-EGF antibody [EPR14622] (ab206106)
    Anti-pro-EGF antibody [EPR14622] (ab206106) at 1/2000 dilution + Human urine sample at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 134 kDa
    Observed band size: 160 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-pro-EGF antibody [EPR14622] (ab206106)
    Immunoprecipitation - Anti-pro-EGF antibody [EPR14622] (ab206106)

    EGF was immunoprecipitated from 1mg of Human milk sample with ab206106 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206106 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human milk sample 10ug (Input).

    Lane 2: ab206106 IP in Human milk sample.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206106 in Human milk sample.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-pro-EGF antibody [EPR14622] (ab206106)
    Anti-pro-EGF antibody [EPR14622] (ab206106)

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