Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)
![Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)](https://www.abcam.com/ps/products/247/ab247219/Images/ab247219-406308-anti-pro-caspase-7-antibody-y33-bsa-and-azide-free-western-blot.jpg "Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y33] to pro Caspase-7 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free
See all pro Caspase-7 primary antibodies -
Description
Rabbit monoclonal [Y33] to pro Caspase-7 - BSA and Azide free -
Host species
Rabbit -
Specificity
The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.
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Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247219 is the carrier-free version of ab32067 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247219 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
Y33 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)
This data was developed using ab32067, the same antibody clone in a different buffer formulation.
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Staurosporin treated HAP1 whole cell lysate (20 µg)
Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)
Lane 4: Staurosporin treated CASP7 knockout HAP1 whole cell lysate (20 µg)
Lane 5: HeLa whole cell lysate (20 µg)
Lane 6: Staurosporin treated HeLa whole cell lysate (20 µg)
Lanes 1 - 6: Merged signal (red and green). Green - ab32067 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab32067 was shown to recognize pro Caspase 7 when HAP1 + pro Caspase 7 knockout samples were used, along with additional cross-reactive bands. Wild-type and pro Caspase 7 knockout samples were subjected to SDS-PAGE. ab32067 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)](https://www.abcam.com/ps/products/247/ab247219/Images/ab247219-2-anti-pro-caspase-7-antibody-y33-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)This data was developed using ab32067, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma using ab32067 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Western blot - Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)](https://www.abcam.com/ps/products/247/ab247219/Images/ab247219-397855-anti-pro-caspase-7-antibody-y33-bsa-and-azide-free-western-blot.gif)
Western blot - Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219) -
![Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)](https://www.abcam.com/ps/products/247/ab247219/Images/ab247219-4-benefits-of-recombinant-antibodies.png)
Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)