Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y33] to pro Caspase-7 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free
    See all pro Caspase-7 primary antibodies

  • Description

    Rabbit monoclonal [Y33] to pro Caspase-7 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7. 

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247219 is the carrier-free version of ab32067 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247219 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)
    Western blot - Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)

    This data was developed using ab32067, the same antibody clone in a different buffer formulation.

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)

    Lane 2: Staurosporin treated HAP1 whole cell lysate (20 µg)

    Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)

    Lane 4: Staurosporin treated CASP7 knockout HAP1 whole cell lysate (20 µg)

    Lane 5: HeLa whole cell lysate (20 µg)

    Lane 6: Staurosporin treated HeLa whole cell lysate (20 µg)

    Lanes 1 - 6: Merged signal (red and green). Green - ab32067 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab32067 was shown to recognize pro Caspase 7 when HAP1 + pro Caspase 7 knockout samples were used, along with additional cross-reactive bands. Wild-type and pro Caspase 7 knockout samples were subjected to SDS-PAGE. ab32067 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)
    This data was developed using ab32067, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma using ab32067 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)
    Western blot - Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)
    Anti-pro Caspase-7 antibody [Y33] (ab32067) at 1/1000 dilution + Jurkat cell lysate

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    This data was developed using ab32067, the same antibody clone in a different buffer formulation.

     

  • Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)
    Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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