Anti-Caspase-11 antibody [EPR22717-11] (ab246496)
![Anti-Caspase-11 antibody [EPR22717-11] (ab246496)](https://www.abcam.com/ps/products/246/ab246496/Images/ab246496-454986-anti-caspase11-antibody-epr2271711-western-blot-with.jpg "Anti-Caspase-11 antibody [EPR22717-11] (ab246496)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22717-11] to Caspase-11
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Overview
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Product name
Anti-Caspase-11 antibody [EPR22717-11]
See all Caspase-11 primary antibodies -
Description
Rabbit monoclonal [EPR22717-11] to Caspase-11 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC or IHC-P -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type iBMDM treated with /ml LPS.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22717-11 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspase-11 antibody [EPR22717-11] (ab246496)All lanes : Anti-Caspase-11 antibody [EPR22717-11] (ab246496) at 1/1000 dilution
Lane 1 : Wild-type iBMDM treated with /ml LPS(Lipopolysaccharide) for 4 hours, whole cell lysate
Lane 2 : Caspase-11 knockout iBMDM treated with /ml LPS(Lipopolysaccharide) for 4 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Observed band size: 43 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
This product is specific to full length Caspase-11.
The image was kindly provided by the collaborator.
ab246496 Anti-Caspase-11 antibody [EPR22717-11] was shown to react with Caspase-11 in iBMDM cells in Western blot. Loss of signal was observed when Caspase-11 knockout sample was used. Wild-type and Caspase-11 knockout samples were subjected to SDS-PAGE. ab246496 was incubated 1 hour at room temperature at 1/1000 dilution . Blots were developed with Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/5000 dilution for 1 hour at room temperature before imaging. Anti-beta Tubulin was used as loading control.
Exposure time: 1 minute
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![Western blot - Anti-Caspase-11 antibody [EPR22717-11] (ab246496)](https://www.abcam.com/ps/products/246/ab246496/Images/ab246496-454987-anti-caspase11-antibody-epr2271711-western-blot-cell.jpg)
Western blot - Anti-Caspase-11 antibody [EPR22717-11] (ab246496)All lanes : Anti-Caspase-11 antibody [EPR22717-11] (ab246496) at 1/1000 dilution
Lane 1 : Wild-type iBMDM whole cell lysate
Lane 2 : Wild-type iBMDM treated with /ml LPS(Lipopolysaccharide) for 4 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution
Observed band size: 43 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
This product is specific to full length.
The lysates were kindly provided by the collaborator.
Exposure time: 3 minutes
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![Anti-Caspase-11 antibody [EPR22717-11] (ab246496)](https://www.abcam.com/ps/products/246/ab246496/Images/ab246496-455245-benefits-of-recombinant-antibodies.jpg)
Anti-Caspase-11 antibody [EPR22717-11] (ab246496)