Anti-Prickle 2 antibody (ab65964)
Key features and details
- Rabbit polyclonal to Prickle 2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Prickle 2 antibody
See all Prickle 2 primary antibodies -
Description
Rabbit polyclonal to Prickle 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Prickle 2 aa 700-800 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90635) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: Y79; WERI.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa).Target
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Tissue specificity
Expressed in brain, eye and testis. Additionally in fetal brain, adult cartilage, pancreatic islet, gastric cancer and uterus tumors. -
Involvement in disease
Epilepsy, progressive myoclonic 5 -
Sequence similarities
Belongs to the prickle / espinas / testin family.
Contains 3 LIM zinc-binding domains.
Contains 1 PET domain. -
Cellular localization
Nucleus membrane. - Information by UniProt
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Database links
- Entrez Gene: 166336 Human
- Entrez Gene: 243548 Mouse
- Omim: 608501 Human
- SwissProt: Q7Z3G6 Human
- SwissProt: Q80Y24 Mouse
- Unigene: 148105 Human
- Unigene: 699317 Human
- Unigene: 248820 Mouse
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Alternative names
- EPM5 antibody
- PRIC2 antibody
- PRIC2_HUMAN antibody
see all
Images
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Western blot - Anti-Prickle 2 antibody (ab65964)All lanes : Anti-Prickle 2 antibody (ab65964) at 1 µg/ml
Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 96 kDa
Observed band size: 96 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Datasheets and documents
References (0)
ab65964 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Prickle 2 antibody (ab65964)All lanes : Anti-Prickle 2 antibody (ab65964) at 1 µg/ml
Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 96 kDa
Observed band size: 96 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
