Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SNAIL antibody [EPR21043] (ab216347)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21043] to SNAIL
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-SNAIL antibody [EPR21043]
    See all SNAIL primary antibodies

  • Description

    Rabbit monoclonal [EPR21043] to SNAIL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human SNAIL recombinant protein (aa1-264); HeLa and HCT 116 whole cell lysates. IP: HeLa whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-SNAIL antibody [EPR21043] (ab216347)
    Immunoprecipitation - Anti-SNAIL antibody [EPR21043] (ab216347)

    SNAIL was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab216347 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216347 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).
    Lane 2: ab216347 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216347 in HeLa whole cell lysate.

    Exposure time: 10 seconds.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-SNAIL antibody [EPR21043] (ab216347)
    Western blot - Anti-SNAIL antibody [EPR21043] (ab216347)
    All lanes : Anti-SNAIL antibody [EPR21043] (ab216347) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    SNAIL expression is not detectable in MCF7 cells, which is consistent with what has been described in the literature (PMID: 10655587 and 22028892).

  • Western blot - Anti-SNAIL antibody [EPR21043] (ab216347)
    Western blot - Anti-SNAIL antibody [EPR21043] (ab216347)
    All lanes : Anti-SNAIL antibody [EPR21043] (ab216347) at 1/10000 dilution

    Lane 1 : His-tagged human SNAIL recombinant protein (aa1-264)
    Lane 2 : His-tagged human Slug recombinant protein (aa21-268)
    Lane 3 : His-tagged human Slug recombinant protein (aa1-110)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-SNAIL antibody [EPR21043] (ab216347)
    Anti-SNAIL antibody [EPR21043] (ab216347)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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