Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SNAIL antibody [EPR21043] (ab216347)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21043] to SNAIL
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-SNAIL antibody [EPR21043]
See all SNAIL primary antibodies
Description

Rabbit monoclonal [EPR21043] to SNAIL
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged human SNAIL recombinant protein (aa1-264); HeLa and HCT 116 whole cell lysates. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21043
Isotype

IgG
Research areas

Immunoprecipitation - Anti-SNAIL antibody [EPR21043] (ab216347)

SNAIL was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab216347 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216347 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: ab216347 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216347 in HeLa whole cell lysate.

Exposure time: 10 seconds.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-SNAIL antibody [EPR21043] (ab216347)

All lanes : Anti-SNAIL antibody [EPR21043] (ab216347) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

SNAIL expression is not detectable in MCF7 cells, which is consistent with what has been described in the literature (PMID: 10655587 and 22028892).
Western blot - Anti-SNAIL antibody [EPR21043] (ab216347)

All lanes : Anti-SNAIL antibody [EPR21043] (ab216347) at 1/10000 dilution

Lane 1 : His-tagged human SNAIL recombinant protein (aa1-264)
Lane 2 : His-tagged human Slug recombinant protein (aa21-268)
Lane 3 : His-tagged human Slug recombinant protein (aa1-110)

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-SNAIL antibody [EPR21043] (ab216347)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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