Anti-PRDM14 antibody (ab187881)
Key features and details
- Rabbit polyclonal to PRDM14
- Suitable for: ICC/IF, ChIP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRDM14 antibody -
Description
Rabbit polyclonal to PRDM14 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ChIP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PRDM14.
Database link: Q9GZV8 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab187881. Blue DAPI for nuclear staining.
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All lanes : Anti-PRDM14 antibody (ab187881) at 1/500 dilution
Lane 1 : BT474 cell extract
Lane 2 : Jurkat cell extract
Lane 3 : HeLa cell extract
Predicted band size: 64 kDa
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All lanes : Anti-PRDM14 antibody (ab187881) at 1/500 dilution
Lane 1 : shRNA-6
Lane 2 : Control
Lane 3 : shRNA-5
Lane 4 : shRNA-4
Lane 5 : shRNA-3
Lane 6 : shRNA-2
Lane 7 : shRNA-1
Predicted band size: 64 kDaWestern blot analysis of various groups from PA-1 cell line. ShRNA-1, shRNA-2, shRNA-3, shRNA-4, shRNA-5, shRNA-6 show knock-down samples which are transfected by different shRNAs targeting PRDM14. Control represents the untreated one.
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Chromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using ab187881 and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.