Anti-PPP2R5C antibody (ab264338)
Key features and details
- Rabbit polyclonal to PPP2R5C
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPP2R5C antibody
See all PPP2R5C primary antibodies -
Description
Rabbit polyclonal to PPP2R5C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PPP2R5C aa 490-524. The exact sequence is proprietary. NP_002710.2
Database link: Q13362 -
Positive control
- IHC-P: Human breast carcinoma tissue; IP: HeLa whole cell lysate; WB: HeLa, HEK-293T and Jurkat whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPP2R5C antibody (ab264338)All lanes : Anti-PPP2R5C antibody (ab264338) at 0.04 µg/ml
Lane 1 : HEK-293T whole cell lysate at 50 µg
Lane 2 : HEK-293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes
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Immunoprecipitation - Anti-PPP2R5C antibody (ab264338)PPP2R5C was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264338 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264338 at 1 µg/ml.
Lane 1: ab264338 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 30 secs.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5C antibody (ab264338)Immunohistochemical analysis of FFPE human breast carcinoma tissue section labeling PPP2R5C with ab264338 at 1/1000 dilution. Hematoxylin was used as counterstain. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
