Anti-PPM1G antibody [EPR13362-31] (ab186423)
![Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.com/ps/products/186/ab186423/Images/ab186423-219982-anti-ppm1g-antibody-epr13362-31-western-blot.jpg "Anti-PPM1G antibody [EPR13362-31] (ab186423)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13362-31] to PPM1G
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PPM1G antibody [EPR13362-31]
See all PPM1G primary antibodies -
Description
Rabbit monoclonal [EPR13362-31] to PPM1G -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P MouseHumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, Jurkat, HeLa and MCF7 cell lysates; Human kidney clear cell carcinoma and Mouse cardiac muscle tissues; HeLa, 293T and 293 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13362-31 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPM1G antibody [EPR13362-31] (ab186423)All lanes : Anti-PPM1G antibody [EPR13362-31] (ab186423) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 59 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.com/ps/products/186/ab186423/Images/ab186423-219979-anti-ppm1g-antibody-epr13362-31-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody [EPR13362-31] (ab186423)Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling PPM1G with ab186423 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.com/ps/products/186/ab186423/Images/ab186423-219978-anti-ppm1g-antibody-epr13362-31-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody [EPR13362-31] (ab186423)Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling PPM1G with ab186423 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.com/ps/products/186/ab186423/Images/ab186423-219977-anti-ppm1g-antibody-epr13362-31-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PPM1G antibody [EPR13362-31] (ab186423)Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PPM1G with ab186423 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (Green). Counter stained with Dapi (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.com/ps/products/186/ab186423/Images/ab186423-219976-anti-ppm1g-antibody-epr13362-31-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PPM1G antibody [EPR13362-31] (ab186423)Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling PPM1G with ab186423 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.
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![Flow Cytometry - Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.com/ps/products/186/ab186423/Images/ab186423-219975-anti-ppm1g-antibody-epr13362-31-flow-cytometry.jpg)
Flow Cytometry - Anti-PPM1G antibody [EPR13362-31] (ab186423)Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling PPM1G with ab186423 at 1/200 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Anti-PPM1G antibody [EPR13362-31] (ab186423)](https://www.abcam.com/ps/products/186/ab186423/Images/ab186423-7-benefits-of-recombinant-antibodies.png)
Anti-PPM1G antibody [EPR13362-31] (ab186423)
