Anti-PPM1E antibody [EPR8721] (ab137122)
![Anti-PPM1E antibody [EPR8721] (ab137122)](https://www.abcam.com/ps/products/137/ab137122/Images/PPM1E-Primary-antibodies-ab137122-1.jpg "Anti-PPM1E antibody [EPR8721] (ab137122)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8721] to PPM1E
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-PPM1E antibody [EPR8721]
See all PPM1E primary antibodies -
Description
Rabbit monoclonal [EPR8721] to PPM1E -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human PPM1E aa 200-300. The exact sequence is proprietary.
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Positive control
- SH-SY5Y, Human hypothalamus, and Human hippocampus lysates, Human brain tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8721 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPM1E antibody [EPR8721] (ab137122)All lanes : Anti-PPM1E antibody [EPR8721] (ab137122) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Human hypothalamus lysate
Lane 3 : Human hippocampus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 84 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1E antibody [EPR8721] (ab137122)](https://www.abcam.com/ps/products/137/ab137122/Images/PPM1E-Primary-antibodies-ab137122-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1E antibody [EPR8721] (ab137122)Immunohistochemical analysis of paraffin embedded Human brain tissue labelling PPM1E with ab137122 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-PPM1E antibody [EPR8721] (ab137122)](https://www.abcam.com/ps/products/137/ab137122/Images/ab137122-3-benefits-of-recombinant-antibodies.png)
Anti-PPM1E antibody [EPR8721] (ab137122)
