Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PP2A alpha + beta antibody [Y119] - BSA and Azide free (ab247238)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y119] to PP2A alpha + beta - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PP2A alpha + beta antibody [Y119] - BSA and Azide free
    See all PP2A alpha + beta primary antibodies

  • Description

    Rabbit monoclonal [Y119] to PP2A alpha + beta - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects both alpha and beta isoforms of PP2A.

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    ab247238 reacts with an epitope located in the C terminal region of PP2A alpha and beta

  • General notes

    Ab247238 is the carrier-free version of ab32141. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247238 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PP2A alpha + beta antibody [Y119] - BSA and Azide free (ab247238)
    Western blot - Anti-PP2A alpha + beta antibody [Y119] - BSA and Azide free (ab247238)
    Anti-PP2A alpha + beta antibody [Y119] (ab32141) at 1/10000 dilution + T47-D cell lysate

    Predicted band size: 35 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32141, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [Y119] - BSA and Azide free (ab247238)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [Y119] - BSA and Azide free (ab247238)
    This data was developed using ab32141, the same antibody clone in a different buffer formulation.ab32141,at a 1/250 dilution, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-PP2A alpha + beta antibody [Y119] - BSA and Azide free (ab247238)
    Flow Cytometry - Anti-PP2A alpha + beta antibody [Y119] - BSA and Azide free (ab247238)
    This data was developed using ab32141, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling PP2A alpha + beta (red) with unpurified ab32141 at dilution of 1/20. Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibodies.
  • Anti-PP2A alpha + beta antibody [Y119] - BSA and Azide free (ab247238)
    Anti-PP2A alpha + beta antibody [Y119] - BSA and Azide free (ab247238)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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