Anti-POLR3A antibody (ab247007)
Key features and details
- Rabbit polyclonal to POLR3A
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-POLR3A antibody
See all POLR3A primary antibodies -
Description
Rabbit polyclonal to POLR3A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chicken, Cow
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Immunogen
Recombinant fragment corresponding to Human POLR3A aa 600-700.
Database link: O14802 -
Positive control
- WB: PC3 and MCF7 whole cell lysates. IHC-P: Human cerebral cortex, testis, placenta and liver tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-POLR3A antibody (ab247007)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for POLR3A (green) using ab247007 at 4 μg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR3A antibody (ab247007)Paraffin-embedded human liver tissue stained for POLR3A using ab247007 at 1/200 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR3A antibody (ab247007)Paraffin-embedded human placenta tissue stained for POLR3A using ab247007 at 1/200 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR3A antibody (ab247007)Paraffin-embedded human testis tissue stained for POLR3A using ab247007 at 1/200 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR3A antibody (ab247007)Paraffin-embedded human cerebral cortex tissue stained for POLR3A using ab247007 at 1/200 dilution in immunohistochemical analysis. -
Western blot - Anti-POLR3A antibody (ab247007)All lanes : Anti-POLR3A antibody (ab247007) at 0.4 µg/ml
Lane 1 : PC-3 whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 156 kDaCorresponding POLR3A RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
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Western blot - Anti-POLR3A antibody (ab247007)Anti-POLR3A antibody (ab247007) at 0.4 µg/ml + PC-3 whole cell lysate
Predicted band size: 156 kDa