Anti-POLR1A antibody (ab241955)
Key features and details
- Rabbit polyclonal to POLR1A
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-POLR1A antibody
See all POLR1A primary antibodies -
Description
Rabbit polyclonal to POLR1A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human POLR1A aa 1350-1400. The exact sequence is proprietary.
Database link: O95602 -
Positive control
- IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to POLR1A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-POLR1A antibody (ab241955)
POLR1A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241955 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using a different POLR1A antibody at 0.4 µg/ml.
Lane 1: ab241955 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 seconds.
Lysates prepared in NETN buffer.
