Anti-POLR1A antibody (ab241950)
Key features and details
- Rabbit polyclonal to POLR1A
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-POLR1A antibody
See all POLR1A primary antibodies -
Description
Rabbit polyclonal to POLR1A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human POLR1A aa 1425-1475. The exact sequence is proprietary.
Database link: O95602 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: POLR1A IP in HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to POLR1A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-POLR1A antibody (ab241950)All lanes : Anti-POLR1A antibody (ab241950) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 3 minutesPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-POLR1A antibody (ab241950)POLR1A was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241950 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.
Lane 1: ab241950 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 3 seconds.
