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Neuroscience Neurotransmitter Biogenic Amines Noradrenaline

Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free (ab249438)

Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free (ab249438)
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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7547(2)] to PNMT - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free
    See all PNMT primary antibodies
  • Description

    Rabbit monoclonal [EPR7547(2)] to PNMT - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249438 is the carrier-free version of ab167427.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7547(2)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Adrenaline
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Catecholamines
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis

Images

  • Western blot - Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free (ab249438)
    Western blot - Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free (ab249438)
    All lanes : Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution

    Lane 1 : K562 lysate
    Lane 2 : Human adrenal gland lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 31 kDa



    This data was developed using ab167427, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free (ab249438)
    Immunocytochemistry - Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free (ab249438)
    This data was developed using ab167427, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of K562 cells, labeling PNMT using ab167427 at a 1/50 dilution.
  • Western blot - Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free (ab249438)
    Western blot - Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free (ab249438)
    Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution + Mouse kidney tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa


    Exposure time: 3 minutes


    This data was developed using ab167427, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free (ab249438)
    Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free (ab249438)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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