Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)-25] - BSA and Azide free (ab256316)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3664(2)-25] to HIF Prolyl Hydroxylases - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)-25] - BSA and Azide free
    See all HIF Prolyl Hydroxylases primary antibodies

  • Description

    Rabbit monoclonal [EPR3664(2)-25] to HIF Prolyl Hydroxylases - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, IP, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cerebrum and lung cancer tissue Flow cyt: HEK-293 cells IP: HEK-293 whole cell lysate WB: HEK-293 and HeLa whole cell lysates

  • General notes

    Ab256316 is the carrier-free version of ab255733. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256316 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)-25] - BSA and Azide free (ab256316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)-25] - BSA and Azide free (ab256316)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling HIF Prolyl Hydroxylases with ab255733 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebrum (PMID: 17726031, 12163023). The section was incubated with ab255733 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255733 ).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)-25] - BSA and Azide free (ab256316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)-25] - BSA and Azide free (ab256316)

    Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling HIF Prolyl Hydroxylases with ab255733 at 1/250 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human lung cancer (PMID: 24382781). The section was incubated with ab255733 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255733 ).

  • Flow Cytometry - Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)-25] - BSA and Azide free (ab256316)
    Flow Cytometry - Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)-25] - BSA and Azide free (ab256316)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293 (human embryonic kidney epithelial cell) cells labelling HIF Prolyl Hydroxylases with ab255733 at 1/400 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255733 ).

  • Immunoprecipitation - Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)-25] - BSA and Azide free (ab256316)
    Immunoprecipitation - Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)-25] - BSA and Azide free (ab256316)

    HIF Prolyl Hydroxylases was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10μg with ab255733 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab255733 1/1000 dilution (0.4 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10μg

    Lane 2: ab255733 IP in HEK-293 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255733 in HEK-293 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255733 ).

  • Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)-25] - BSA and Azide free (ab256316)
    Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)-25] - BSA and Azide free (ab256316)

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