Monoamine Oxidase (MAO) Assay Kit (ab241031)
Key features and details
- Detection method: Fluorescent
- Sample type: Plasma, Serum
Overview
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Product name
Monoamine Oxidase (MAO) Assay Kit -
Detection method
Fluorescent -
Sample type
Serum, Plasma -
Product overview
Monoamine Oxidase (MAO) Assay Kit (ab241031) is a sensitive assay to detect total monoamine oxidase activity as well as MAO-A and MAO-B isoenzyme activities separately in the presence of Clorgyline and Selegiline - specific inhibitors for MAO-A and MAOB, respectively. The assay is based on the fluorometric detection of H2O2, one of the by-products generated during the oxidative deamination of the MAO substrate (Tyramine). The assay can detect as little as 5 µU of MAO enzymatic activity.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Developer (Lyophilized) 1 vial H2O2 Standard (0.88 M) 1 x 100µl MAO Assay Buffer 1 x 25ml MAO Substrate (Lyophilized) 1 vial MAO-A Inhibitor, Clorgyline (Lyophilized) 1 vial MAO-B Inhibitor, Selegiline (Lyophilized) 1 vial Positive Control (MAO-A Enzyme) (Lyophilized) 1 vial Probe (in DMSO) 1 x 220µl -
Research areas
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Relevance
Monoamine oxidases are enzymes that catalyze the oxidation of monoamines. They belong to protein family of flavin containing amine oxidoreductases. In humans there are two types of MAO: MAO-A and MAO-B. Both are found in neurons and astroglia. Outside the central nervous system: MAO-A is also found in the liver, gastrointestinal tract and placenta. MAO-B is mostly found in blood platelets. Because of the vital role that MAOs play in the inactivation of neurotransmitters, MAO dysfunction (too much/too little MAO activity) is thought to be responsible for a number of neurological disorders. -
Cellular localization
Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side. -
Alternative names
- Adrenalin oxidase
- Amine oxidase
- Flavin containing A
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Images
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H2O2 Standard Curve.
H2O2 Standard Curve.
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MAO-T, MAO-A and MAO-B activities in mouse brain lysate (2.5 µg)MAO-T, MAO-A and MAO-B activities in mouse brain lysate (2.5 µg).
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Specific activities of MAO-A and MAO-B. Assays were performed following the kit protocols.Specific activities of MAO-A and MAO-B. Assays were performed following the kit protocols.