Anti-PKC mu/PKD (phospho S738) antibody (ab131101)
Key features and details
- Rabbit polyclonal to PKC mu/PKD (phospho S738)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKC mu/PKD (phospho S738) antibody
See all PKC mu/PKD primary antibodies -
Description
Rabbit polyclonal to PKC mu/PKD (phospho S738) -
Host species
Rabbit -
Specificity
ab131101 detects endogenous levels of PKC mu/PKD only when phosphorylated at serine 738.
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Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human PKC mu/PKD conjugated to keyhole limpet haemocyanin.
Sequence:E-K-S(p)-F-R
Database link: Q15139 -
Positive control
- MCF cell extract untreated and treated with PMA; HeLa cells
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General notes
This product was previously labelled as PKC mu
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131101 was purified by affinity-chromatography using epitope-specific phosphopetide. Non-phospho specific antibodies were removed by chromatography using non-phosphopetide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PKC mu/PKD (phospho S738) antibody (ab131101)All lanes : Anti-PKC mu/PKD (phospho S738) antibody (ab131101) at 1/500 dilution
Lane 1 : MCF cell extract untreated
Lane 2 : MCF cell extract treated with PMA
Predicted band size: 102 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-PKC mu/PKD (phospho S738) antibody (ab131101)Immunofluorescence analysis of methanol-fixed HeLa cells labelling PKC mu/PKD (phospho S738) with ab131101 at 1/100 dilution.
