Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17074] to PKC delta + PKC theta
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PKC delta + PKC theta antibody [EPR17074]
    See all PKC delta + PKC theta primary antibodies

  • Description

    Rabbit monoclonal [EPR17074] to PKC delta + PKC theta

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Active Human PKC delta full length protein and Active Human PKC theta full length protein. Mouse and Rat thymus lysates. Molt4, HeLa and Raw264.7 cell lysates; Human fetal brain lysates; Rat and mouse brain lysates. IHC-P: Human tonsil and human breast cancer, mouse kidney, rat spleen tissues. ICC/IF: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17074

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    All lanes : Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127) at 1/2000 dilution

    Lane 1 : Active human PKC alpha full length protein
    Lane 2 : Active human PKC beta 1 full length protein
    Lane 3 : Active human PKC beta 2 full length protein
    Lane 4 : Active human PKC gamma full length protein
    Lane 5 : Active human PKC delta full length protein
    Lane 6 : Active human PKC eta full length protein
    Lane 7 : Active human PKC epsilon full length protein
    Lane 8 : Active human PKC theta full length protein
    Lane 9 : Active human PKC mu full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78, 82 kDa


    Exposure time: 5 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

    Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein (ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag; Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag; Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.

  • Immunocytochemistry/ Immunofluorescence - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    Immunocytochemistry/ Immunofluorescence - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PKC delta + PKC theta with ab182127 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing both cytoplasm and nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab182127 at 1/200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PKC delta + PKC theta with ab182127 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on lymphocytes on germinal center of Human tonsil is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    All lanes : Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127) at 1/2000 dilution

    Lane 1 : Mouse thymus lysates
    Lane 2 : Rat thymus lysates
    Lane 3 : Molt4 (Human lymphoblastic leukemia cell line) whole cell lysates
    Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 78, 82 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?



    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127) at 1/2000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 78, 82 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?



    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    All lanes : Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127) at 1/10000 dilution

    Lane 1 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 2 : Rat brain lysates
    Lane 3 : Mouse brain lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 78, 82 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?



    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling PKC delta + PKC theta with ab182127 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on cancer cells of Human breast carcinoma is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)

    Immunohistochemical analysis of paraffin-embedded mouse kidney cancer tissue labeling PKC delta + PKC theta with ab182127 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse kidney tubules is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)

    Immunohistochemical analysis of paraffin-embedded rat spleen cancer tissue labeling PKC delta + PKC theta with ab182127 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on monocytes and macrophages on rat spleen is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)
    Anti-PKC delta + PKC theta antibody [EPR17074] (ab182127)

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