Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP380Y] to PI 3 Kinase p85 alpha - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free
    See all PI 3 Kinase p85 alpha primary antibodies

  • Description

    Rabbit monoclonal [EP380Y] to PI 3 Kinase p85 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A431, MCF7 or Raji cells lysate, HeLa cells, breast carcinoma

  • General notes

    Ab182617 is the carrier-free version of ab40755. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab182617 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617)
    Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617)

    This data was developed using ab40755, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: PI 3 Kinase p85 alpha knockout HAP1 cell lysate (20 µg)

    Lane 3: Jurkat cell lysate (20 µg)

    Lane 4: THP1 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab40755 observed at 90 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab40755 was shown to recognize PI 3 Kinase p85 alpha in wild-type HAP1 cells along with additional cross-reactive bands. No Band was observed when PI 3 Kinase p85 alpha knockout samples were examined. Wild-type and PI 3 Kinase p85 alpha knockout samples were subjected to SDS-PAGE. ab40755 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617)
    Western blot - Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617)
    All lanes : Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755) at 1/2000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 85 kDa
    Observed band size: 85 kDa



    This data was developed using ab40755, the same antibody clone in a different buffer formulation.

  • Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617)
    Anti-PI 3 Kinase p85 alpha antibody [EP380Y] - BSA and Azide free (ab182617)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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