Anti-Phosphotyrosine antibody [Py72] (ab269515)
![Anti-Phosphotyrosine antibody [Py72] (ab269515)](https://www.abcam.com/ps/products/269/ab269515/Images/ab269515-395271-anti-phosphotyrosine-antibody-py72-western-blot-nih3t3.jpg "Anti-Phosphotyrosine antibody [Py72] (ab269515)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [Py72] to Phosphotyrosine
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Phosphotyrosine antibody [Py72]
See all Phosphotyrosine primary antibodies -
Description
Mouse monoclonal [Py72] to Phosphotyrosine -
Host species
Mouse -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Other Immunogen Type. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Pervanadate treated NIH/3T3, Daudi and MCF7 whole cell lysates. IP: Pervanadate treated NIH/3T3 and MCF7 whole cell lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Py72 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Phosphotyrosine antibody [Py72] (ab269515)All lanes : Anti-Phosphotyrosine antibody [Py72] (ab269515) at 0.196 µg/ml
Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : NIH/3T3 treated with 10mM pervanadate for 20 minutes, whole cell lysates
Lane 3 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
Lane 4 : Daudi treated with 10mM pervanadate for 30 minutes, whole cell lysates
Lane 5 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 6 : MCF7 treated with 1mM pervanadate for 20 minutes, whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilutionMultiple bands represent phosph-tyrosine containing proteins detected by ab269515.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-4: 3.25 secs; Lanes 5-6: 3 mins.
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![Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] (ab269515)](https://www.abcam.com/ps/products/269/ab269515/Images/ab269515-1-anti-phosphotyrosine-antibody-py72-immunoprecipitation-mcf7.jpg)
Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] (ab269515)Phosphotyrosine was immunoprecipitated from MCF7 (human breast adenocarcinoma epithelial cell), treated with 1mM pervanadate for 20 minutes, whole cell lysates, with ab269515 at 1/30 dilution (2µg in 0.35mg lysates, (32.667µg/ml)). Western blot was performed on the immunoprecipitate using ab269515 at 1/1000 dilution (0.196µg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MCF7 treated with 1mM pervanadate for 20 mins, whole cell lysates, 10µg.
Lane 2: ab269515 IP in MCF7 treated with 1mM pervanadate for 20 mins, whole cell lysates.
Lane 3: Mouse monoclonal IgG1 (ab18443) instead of ab269515 in MCF7 treated with 1mM pervanadate for 20 mins, whole cell lysates.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Multiple bands represent phosph-tyrosine containing proteins precipitated and detected by ab269515.
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![Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] (ab269515)](https://www.abcam.com/ps/products/269/ab269515/Images/ab269515-2-anti-phosphotyrosine-antibody-py72-immunoprecipitation-nih3t3.jpg)
Immunoprecipitation - Anti-Phosphotyrosine antibody [Py72] (ab269515)Phosphotyrosine was immunoprecipitated from NIH/3T3 (Mouse embyro fibroblast cells), treated with 10mM pervanadate for 20 minutes, whole cell lysates, with ab269515 at 1/30 dilution (2µg in 0.35mg lysates, (32.667µg/ml)). Western blot was performed on the immunoprecipitate using ab269515 at 1/1000 dilution (0.196µg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 treated with 10mM pervanadate for 20 mins, whole cell lysates, 10µg.
Lane 2: ab269515 IP in NIH/3T3 treated with 10mM pervanadate for 20 mins, whole cell lysates.
Lane 3: Mouse monoclonal IgG1 (ab18443) instead of ab269515 in NIH/3T3 treated with 10mM pervanadate for 20 mins, whole cell lysates.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Multiple bands represent phosph-tyrosine containing proteins precipitated and detected by ab269515.
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![Anti-Phosphotyrosine antibody [Py72] (ab269515)](https://www.abcam.com/ps/products/269/ab269515/Images/ab269515-4-benefits-of-recombinant-antibodies.png)
Anti-Phosphotyrosine antibody [Py72] (ab269515)