Anti-PGP9.5 antibody - Neuronal Marker (ab15503)
Key features and details
- Rabbit polyclonal to PGP9.5 - Neuronal Marker
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PGP9.5 antibody - Neuronal Marker
See all PGP9.5 primary antibodies -
Description
Rabbit polyclonal to PGP9.5 - Neuronal Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human PGP9.5.
Database link: P09936 -
Positive control
- Pancreas and small intestine This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody - Neuronal Marker (ab15503)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for PGP9.5 using ab15503 at 1/200 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody - Neuronal Marker (ab15503)ICC/IF image of ab15503 stained DU145. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15503 at 1 ug/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-PGP9.5 antibody - Neuronal Marker (ab15503)Anti-PGP9.5 antibody - Neuronal Marker (ab15503) at 1/25 dilution + Human Brain lysate
Predicted band size: 25 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
