Anti-PEX5/PER3 antibody (ab204444)
Key features and details
- Rabbit polyclonal to PEX5/PER3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PEX5/PER3 antibody -
Description
Rabbit polyclonal to PEX5/PER3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Cow, Chinese hamster -
Immunogen
Recombinant fragment corresponding to Human PEX5/PER3 aa 200-350.
Database link: P50542 -
Positive control
- IHC-P: Human testis, colon, lymph node, cerebral cortex, and pancreas tissues; ICC: A431 cells; WB: RT-4, U-251 MG, and U-2 OS human cell lysates.
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General notes
This product was previously labelled as PEX5
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling PEX5/PER3 with ab204444 at 1/50 dilution.
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Immunocytochemistry analysis of A431 (human epidermoid carcinoma) cells labelling PEX5/PER3 with ab204444 at 2 µg/mL.
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All lanes : Anti-PEX5/PER3 antibody (ab204444) at 0.4 µg/ml
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) transfected with control siRNA, cell lysate
Lane 2 : U-2 OS transfected with target specific siRNA probe #1, cell lysate
Lane 3 : U-2 OS transfected with target specific siRNA probe #2, cell lysate
Predicted band size: 71 kDa
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All lanes : Anti-PEX5/PER3 antibody (ab204444) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cell line) cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) cell lysate
Predicted band size: 71 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling PEX5/PER3 with ab204444 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling PEX5/PER3 with ab204444 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling PEX5/PER3 with ab204444 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling PEX5/PER3 with ab204444 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.