Anti-PEX14 antibody (ab183885)
Key features and details
- Rabbit polyclonal to PEX14
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PEX14 antibody
See all PEX14 primary antibodies -
Description
Rabbit polyclonal to PEX14 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human PEX14 aa 174-377. The exact sequence is proprietary.
Database link: O75381 -
Positive control
- WB: HEK-293T, A431, HeLa, Neuro-2a, C8-D30, NIH/3T3, RAW 264.7, C2C12, PC-12, Rat2 whole cell lysate; IF: Hela whole cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PEX14 antibody (ab183885)All lanes : Anti-PEX14 antibody (ab183885) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 41 kDa10% SDS PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-PEX14 antibody (ab183885)Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cells labeling PEX14 with ab183885 at 1/500 dilution (green) and Hoechst 33342 (blue). Cells were fixed in 4% paraformaldehyde at room temperature for 15 mins.
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Western blot - Anti-PEX14 antibody (ab183885)All lanes : Anti-PEX14 antibody (ab183885) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 41 kDa10% SDS-PAGE
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Western blot - Anti-PEX14 antibody (ab183885)All lanes : Anti-PEX14 antibody (ab183885) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 41 kDa10% SDS-PAGE
