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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8115] to Peroxiredoxin 3/PRDX3 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, ICC, Flow Cyt
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free
    See all Peroxiredoxin 3/PRDX3 primary antibodies
  • Description

    Rabbit monoclonal [EPR8115] to Peroxiredoxin 3/PRDX3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, 293T, MCF7 and A431 cell lysates
      IHC-P: Human endometrium adenocarcinoma and Human liver tissues
  • General notes

    ab234918 is the carrier-free version of ab128953 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab234918 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Peroxiredoxin 3

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 1.02 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8115
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Heart disease

Images

  • Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)
    Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)

    This data was developed using ab128953, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Peroxiredoxin 3/PRDX3 knockout HAP1 cell lysate (20 µg)

    Lane 3: MCF7 cell lysate (20 µg)

    Lane 4: A431 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab128953 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

     

    ab128953 was shown to specifically react with Peroxiredoxin 3/PRDX3 in wild-type HAP1 cells. No band was observed when Peroxiredoxin 3/PRDX3 knockout samples were examined. Wild-type and Peroxiredoxin 3/PRDX3 knockout samples were subjected to SDS-PAGE. ab128953 and ab8245 (loading control to GAPDH) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)
    This data was developed using ab128953, the same antibody clone in a different buffer formulation.ab128953, at a 1/250 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)
    Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)
    All lanes : Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 28 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab128953, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)
    This data was developed using ab128953, the same antibody clone in a different buffer formulation.ab128953, at a 1/250 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin embedded Human liver tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)
    OI-RD Scanning - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)
    This data was developed using ab128953, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)
    Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] - BSA and Azide free (ab234918)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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