Anti-PDZ-RhoGEF antibody (ab223714)
Key features and details
- Rabbit polyclonal to PDZ-RhoGEF
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDZ-RhoGEF antibody
See all PDZ-RhoGEF primary antibodies -
Description
Rabbit polyclonal to PDZ-RhoGEF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human PDZ-RhoGEF aa 350-550.
Database link: O15085 -
Positive control
- IHC-P: Human testis, colon, lymph node and cerebral cortex tissue. WB: ARHGEF11 over-expression HEK-293T whole cell lysates.
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General notes
Previously labelled as ARHGEF11.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDZ-RhoGEF antibody (ab223714)All lanes : Anti-PDZ-RhoGEF antibody (ab223714) at 0.4 µg/ml
Lane 1 : Only vector transfected - HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : ARHGEF11 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 168 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZ-RhoGEF antibody (ab223714)Immunohistochemical analysis of human testis tissue labeling PDZ-RhoGEF with ab223714 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZ-RhoGEF antibody (ab223714)Immunohistochemical analysis of human colon tissue labeling PDZ-RhoGEF with ab223714 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZ-RhoGEF antibody (ab223714)Immunohistochemical analysis of human lumph node tissue labeling PDZ-RhoGEF with ab223714 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZ-RhoGEF antibody (ab223714)Immunohistochemical analysis of human cerebral cortex tissue labeling PDZ-RhoGEF with ab223714 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
